iMAPS Workshop: Single-Cell In-situ Metabolism–Targeted Isolation of Uncultured Microbes
This iMAPS Workshop, jointly organized by the Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (QIBEBT, CAS), the BISMiS 2025 organizing committee, and Qingdao Single-Cell Biotech. co., ltd., focuses on how to directly detect, sort, and cultivate high-value functional microbes that have never been cultured before.
The session will highlight a full pipeline that links in-situ single-cell metabolic readout to live isolation, cultivation, and verification, aiming at real applications in biomanufacturing, environmental sustainability, and health.
Workshop Content
How label-free single-cell Raman spectra can read each cell’s “metabolic fingerprint” directly in complex communities, without fluorescent labeling or pre-culturing. This enables large-scale, automated, low-cost functional profiling to support biomanufacturing, medical health, environmental monitoring, and biosafety.
High-throughput FlowRACS “screen-then-culture” strategy
FlowRACS is a Raman flow sorter that uses technologies such as pDEP-DLD to keep signal quality stable while running continuously at ≥600 events/min. It can enrich fast-growing, high-producing, or stress-tolerant target cells from complex microbiomes or large mutant libraries.
Digital Colony Picker (DCP) and AI-guided isolation
Who Should Attend
Researchers in microbiology, synthetic biology, and biomanufacturing who need to rapidly discover robust, high-performance strains — especially rare or uncultured microbes from real environments — and move them toward engineering and industrial use.
